Optimization of Extraction Process of Thymol and Carvacrol in Origanum vulgare by Box-Behnken Design-Response Surface Methodology / 中国药学杂志

OBJECTIVE: To optimize the extraction process of thymol and carvacrol in Origanum vulgare by Box-Behnken design-response surface methodology. METHODS: On the basis of single factor experiment, taking the sum of extraction rates of thymol and carvicol as the evaluation index, Box-Behnken design was used to investigate the effects of ethanol concentration, liquid-solid ratio and medicinal powder on the extraction rate. RESULTS: The optimal extraction parameters were as follows: ethanol concentration was 80%, liquid to solid ratio was 13∶1 (mL/g), medicinal powder passing through 40 meshes was used, and the highest extraction rate was 694.80 μg•g-1, with a small deviation from the predicted value. CONCLUSION: The optimal extraction method is simple, with low cost and good predictivity, and it can provide experimental basis for further large-scale production of thymol and carvacrol in Origanum vulgare.

Infectious complications in patients with crush syndrome following the Wenchuan earthquake / 中华创伤杂志(英文版)

<p><b>OBJECTIVE</b>To analyze the results of clinical and bacteriological examinations in patients with crush syndrome who suffered infectious complications after an earthquake in Sichuan, China.</p><p><b>METHODS</b>A total of 313 bacteriological samples among 147 patients with crush syndrome were collected. Infectious complications, results of microbiological examinations, potential risk factors of infection and mortality were analyzed statistically.</p><p><b>RESULTS</b>In the obtained database, 112 out of the 147 (75.7%) patients had infectious complications, in which, wound infection, pulmonary infection, and sepsis were most common. The time under the rubble and the time from injury to treatment were related to the occurrence of wound infection (P equal to 0.013, odds ratio 2.25; P equal to 0.017, odds ratio 2.31). Sepsis and wound infection were more common in patients who underwent fasciotomy or amputation than in those who did not (P equal to 0.001).</p><p><b>CONCLUSION</b>Quick rescue and injury treatment can decrease the infection risk in crush syndrome patients. It is better to obtain microorganic proofs before applying antibiotics, and bacteriological and drug sensitivity data should be taken into account, especially considering that most of these infections are hospital-acquired and drug resistance. Emphasizing the accuracy and efficiency of wound management in emergency situations, cautiously assessing the indications for fasciotomy to avoid open wounds from unnecessary osteofascial compartment decompression incisions may decrease the incidence of infection and ameliorate the prognosis.</p>

HPLC characteristic chromatographic profile of Sarcandra glabra / 中国中药杂志

<p><b>OBJECTIVE</b>To develop the characteristic chromatographic profile of Sarcandra glabra by HPLC for its quality control.</p><p><b>METHOD</b>The HPLC analysis was performed on an Agilent Zorbax Eclipse XDB-C18 column (4. 6 mm x 250 mm, 5 microm) with column temperature at 40 degree C. The mobile phase was consisted of water containing 0. 5% formic acid and acetonitrile to methanol (1:9) in gradient mode, and the detection wavelength was set at 344 nm.</p><p><b>RESULT</b>A common mode of the HPLC characteristic chromatographic profile has been establised. There were 20 common peaks , seven of which were identified, and 46 samples from different habitats were classified into five groups based on principal component cluster analysis.</p><p><b>CONCLUSION</b>The method was time-saving and can represent the chemical information and provide a scientific basis for quality control of S. glabra.</p>

Studies on involatile constituents of Mentha haplocalyx / 中国中药杂志

<p><b>OBJECTIVE</b>To promote its comprehensive utilization, the involatile constituents of Mentha haplocalyx were studied systematically.</p><p><b>METHOD</b>The chemical components were isolated and purified by silca gel column chromatography and recrystallization. The chemical structures were elucidated on the basis of physico-chemical properties and spectral data.</p><p><b>RESULT</b>Eight compounds were isolated and identified as: acacetin (I), tilianine (II), linarin (III), n-butyl-beta-D-fructopyranoside (IV), ursolic acid (V), oleanolic acid (VI), beta-sitosterol (VII), daucosterol (VIII).</p><p><b>CONCLUSION</b>Compounds I approximately V were obtained from this plant for the first time.</p>